CRYOLIFE at work

1 Registration

Upon registration with us, each customer will be assigned a barcode label with a registration number for all future identification. We will also provide each customer with a collection kit for their doctor's use.

2 Collection.

After the baby is delivered, your doctor will collect the cord blood remaining in the umbilical vein and placenta using the sterilized closed-system collection bag set we provided. The collection process is user-friendly and painless.

3 Transport to Laboratory.

Within 12 hours after notification from the customer, our dedicated transport staff will arrive the hospital with a temperature-controlled cell safe to collect the kit.

4 Verification of Information and Sample.

At the laboratory, our professional technician will verify the identity information and test the blood specimens for infectious diseases and blood grouping. Before processing, a small cord blood sample will be retrieved for stipulated tests.

5 Automated Processing.

We will remove red blood cells and plasma from the cord blood sample to obtain cord blood stem cells. This process will be performed by an advance automated cell recovery system which is functionally closed and sterile, ensuring effective contamination control and efficient recovery of the mononuclear cells (MNCs) from cord blood. After this process a small concentrated cord blood aliquot is prepared for stipulated tests.

6 Controlled-Rate Freezing and Storage.

After processing, we will add cryoprotectant to the processed cord blood unit and it will be ready for storage. The unit will undergo a slow freezing process at a controlled rate until it reaches -90'C before it is transferred to a vapour phase liquid nitrogen tank for long-term storage at -185 to -190'C.

7 On-going Quality Assurance.

We have put in place a biannual quality assurance program to ensure the good quality of cryopreserved cord blood units in each liquid nitrogen tank. Terminated cryopreserved units will be retrieved and thawed for quality tests including cell viability and cord blood stem cell count. Cord blood stem cell activities are also analyzed by performing colony formation unit (CFU) assay.